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Postdoctoral Position Available in: Functional Genomics of Vascular Disease

PI: Rajat Gupta, MD

Divisions of Genetics and Cardiovascular Medicine

Brigham and Women’s Hospital / Harvard Medical School

Boston, Massachusetts

Preferred start date: November 1, 2019- September 1, 2020.

Please send a current CV, at least 2 letters of reference, and 1-page (maximum) statement of Research Interests and Career Goals to Raj Gupta. We are a fun and inclusive research group, aspiring to produce top science and scientists through attentive mentoring and access the incredible academic environment of Boston, Harvard Medical School, and the Broad Institute.

QUALIFICATION

  • PhD in cell biology, molecular biology, genetics or genomics
  • Prior research experience in at least two of the following areas: vascular biology, epigenetics, cardiovascular physiology, cellular physiology and next-generation genome sequencing assays especially RNA-seq.
  • Experience in single cell technologies for transcriptomics, flow cytometry, cellular histology and confocal microscopy/live-cell imaging
  • Candidates should have a publication record in peer-reviewed journals.
  • Excellent writing and communication skills, able to work independently and effectively with other members in a productive team.
  • Experience with R, Python or similar statistical package is helpful, as well as experience with clustering, pathway, genetic and genomic data analyses and bioinformatics.

SUMMARY

The Gupta lab is seeking an independent, highly-motivated PhD-level scientist (<2 years postdoc experience) with a strong background in cell biology and next-generation sequencing techniques to join several new projects funded by the NIH New Investigator Award DP2 HL152423. This five-year project aims to: 1) identify the full set of pleiotropic vascular disease loci, 2) identify the cell types and cell states in which non-coding variants affect gene expression using single cell RNA and ATAC-sequencing, and 3) directly measure the effect of variants using pooled CRISPR perturbation screens in vascular cells.

The Gupta lab at Harvard Medical School and Brigham and Women’s Hospital is dedicated to finding new mechanisms of vascular diseases such as coronary artery disease and stroke using genetics as a guide. We have successfully used genome-wide association studies, exome sequencing, single cell RNA-sequencing, ATAC-seq, and Perturb-seq methods to better understand the cellular processes that contribute to disease.

The successful candidate will be appointed to Harvard Medical School, Brigham and Women’s Hospital and the Broad Institute of Harvard and MIT. We collaborate will several labs at each institution, and the environment will provide the perfect environment to learn cutting edge skills and launch an independent career.

Technical Research Assistant I

The Gupta Laboratory in the Divisions of Cardiovascular Medicine and Genetics is looking for a self-motivated, curious research assistant interested in using new genomic methods to study vascular disease. The successful candidate will work on projects to understand the cells that cause heart disease using single cell RNA-sequencing methods. We are looking for a team-oriented, creative, bench scientist, able to excel in a fast-track environment. He or she must be detail-oriented and exhibit strong problem-solving skills. Prior experience with molecular biology, RNA-sequencing, and/or cell biology is a plus but candidates at all levels of experience are encouraged to apply.

Mentorship and guidance will be provided by the Principal Investigator (Dr. Gupta) and other members of the lab.

QUALIFICATION

  • BS in a biological/physical science required.

  • Experience with basic molecular biology techniques and prior cell culture experience.
  • A passion for science and sense of urgency to find new medicines to benefit patients.

PRINCIPAL DUTIES AND RESPONSIBILITIES

  1. Under general supervision, performs a variety of routine tests following established methodologies. Routine tests may include cell cultures, PCR, RNA extraction, and next-generation sequencing.
  2. Prepares and maintains tissue cultures. 


  3. Record data, summarizes, and analyzes results using basic statistics.
  4. Prepares laboratory reagents, chemicals, instruments, and equipment.
  5. Maintains supplies inventory in accordance with departmental procedures.
  6. Discusses progress of work regularly with supervisor.
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